Cultivation of Mycobacterium lepraemurium in a Cell-Free Liquid Medium
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منابع مشابه
Quantitative multiplication of Mycobacterium lepraemurium in a cell-free liquid medium (NC-5).
Mycobacterium Zepraemurium multiplies in a cell-free liquid medium, referred to as NC-5, which is enriched Kirchner medium plus goat serum, a-ketoglutaric acid, cytochrome c, haemin, and L-cysteine. At 30 "C, the bacilli gradually elongated before multiplying 100to Iooo-fold. The maximum number of bacilli is reached after 8 weeks' incubation. The generation time of Mycobacterium lepraemurium is...
متن کاملComparative ultrastructure of Mycobacterium leprae and Mycobacterium lepraemurium cell envelopes.
The structural properties of the cell envelopes of Mycobacterium leprae and Mycobacterium lepraemurium were investigated by freeze-fracture, freeze-etching, and negative-staining techniques. Freeze-fracture split the cell wall and exposed the internal features of the peptidoglycolipid mycosidic filamentous network. The cell membrane was also split into two asymmetric faces. The external fractur...
متن کاملApplication of diffusion chamger technic to the cultivation of Mycobacterium lepraemurium. I. In vivo studies.
Numerous studies have so far been conducted on the cultivation of Mycobacterium leprae and Mycobacterium lepraemurium. However, no media-bound method of cultivation has been established. A number of investigators have reported that M. lepraemurium may be cultivated by cell culture technics. The method employed by Chang (1. 2. 3 ), Chang and Neikirk (4), Chang, Anderson and Vaituzis (~), in whic...
متن کاملGrowth of Mycobacterium lepraemurium in Cell-Impermeable Diffusion Chambers.
Successful growth of Mycobacterium lepraemurium has been achieved by use of a specialized diffusion chamber technique. The cell-impermeable porous chambers were maintained in animals for periods up to 50 days with and without macrophages and LM cells. A generation time of 6 to 8 days was found for the acid-fast bacilli in chambers containing macrophages when maintained in the mouse. Also, cell-...
متن کاملA medium for the rapid cultivation of Mycobacterium avium.
The search for chemotherapeutic agents effective against the tubercle bacillus has brought into sharp focus the slow rate of growth of the organism. Dubos (1945), Davis and Dubos (1947), and Dubos and Middlebrook (1948) developed a liquid medium containing sorbitan monooleate ("tween 80") and albumin which supported rapid and uniform growth of tubercle bacilli. However, Long, as far back as 191...
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ژورنال
عنوان ژورنال: Nippon Saikingaku Zasshi
سال: 1974
ISSN: 1882-4110,0021-4930
DOI: 10.3412/jsb.29.517